Wastewater Surviellence for SARS-CoV-2 in Athens, GA
Lipp Laboratory, Department of Environmental Health Science, University of Georgia
2020-11-02
Load all required libraries.
library(tidyverse)## Warning: package 'tidyverse' was built under R version 3.6.3## -- Attaching packages ---------------------------------------------------------------------------- tidyverse 1.3.0 --## v ggplot2 3.3.2 v purrr 0.3.4
## v tibble 3.0.3 v dplyr 1.0.0
## v tidyr 1.1.0 v stringr 1.4.0
## v readr 1.3.1 v forcats 0.5.0## Warning: package 'ggplot2' was built under R version 3.6.3## Warning: package 'tibble' was built under R version 3.6.3## Warning: package 'readr' was built under R version 3.6.3## Warning: package 'dplyr' was built under R version 3.6.3## Warning: package 'forcats' was built under R version 3.6.3## -- Conflicts ------------------------------------------------------------------------------- tidyverse_conflicts() --
## x dplyr::filter() masks stats::filter()
## x dplyr::lag() masks stats::lag()library(plotly)## Warning: package 'plotly' was built under R version 3.6.3##
## Attaching package: 'plotly'## The following object is masked from 'package:ggplot2':
##
## last_plot## The following object is masked from 'package:stats':
##
## filter## The following object is masked from 'package:graphics':
##
## layoutlibrary(broom)## Warning: package 'broom' was built under R version 3.6.3Read in raw data from RDS.
raw_data <- readRDS("./n1_n2_cleaned_cases.rds")Make a few small modifications to names and data for visualizations.
final_data <- raw_data %>% mutate(log_copy_per_L = log10(mean_copy_num_L)) %>%
rename(Facility = wrf) %>%
mutate(Facility = recode(Facility,
"NO" = "WRF A",
"MI" = "WRF B",
"CC" = "WRF C"))Seperate the data by gene target to ease layering in the final plot
#make three data layers
only_positives <<- subset(final_data, (!is.na(final_data$Facility)))
only_n1 <- subset(only_positives, target == "N1")
only_n2 <- subset(only_positives, target == "N2")
only_background <<-final_data %>%
select(c(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke, cases_per_100000_clarke)) %>%
group_by(date) %>% summarise_if(is.numeric, mean)
#specify fun colors
background_color <- "#7570B3"
seven_day_ave_color <- "#E6AB02"
marker_colors <- c("N1" = '#1B9E77',"N2" ='#D95F02')#remove facilty C for now
#only_n1 <- only_n1[!(only_n1$Facility == "WRF C"),]
#only_n2 <- only_n2[!(only_n2$Facility == "WRF C"),]Build the main plot
#first layer is the background epidemic curve
p1 <- only_background %>%
plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~new_cases_clarke,
type = "bar",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Daily Cases: ', new_cases_clarke),
alpha = 0.5,
name = "Daily Reported Cases",
color = background_color,
colors = background_color,
showlegend = FALSE) %>%
layout(yaxis = list(title = "Clarke County Daily Cases", showline=TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#renders the main plot layer two as seven day moving average
p1 <- p1 %>% plotly::add_trace(x = ~date, y = ~X7_day_ave_clarke,
type = "scatter",
mode = "lines",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Seven-Day Moving Average: ', X7_day_ave_clarke),
name = "Seven Day Moving Average Athens",
line = list(color = seven_day_ave_color),
showlegend = FALSE)
#renders the main plot layer three as positive target hits
p2 <- plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n1,
symbol = ~Facility,
marker = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n2,
symbol = ~Facility,
marker = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(yaxis = list(title = "SARS CoV-2 Copies/L",
showline = TRUE,
type = "log",
dtick = 1,
automargin = TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#adds the limit of detection dashed line
p2 <- p2 %>% plotly::add_segments(x = as.Date("2020-03-14"),
xend = ~max(date + 10),
y = 3571.429, yend = 3571.429,
opacity = 0.35,
line = list(color = "black", dash = "dash")) %>%
layout(annotations = list(x = as.Date("2020-03-28"), y = 3.8, xref = "x", yref = "y",
text = "Limit of Detection", showarrow = FALSE))
p1## Warning: `arrange_()` is deprecated as of dplyr 0.7.0.
## Please use `arrange()` instead.
## See vignette('programming') for more help
## This warning is displayed once every 8 hours.
## Call `lifecycle::last_warnings()` to see where this warning was generated.## Warning: Ignoring 1 observations p2## Warning: `group_by_()` is deprecated as of dplyr 0.7.0.
## Please use `group_by()` instead.
## See vignette('programming') for more help
## This warning is displayed once every 8 hours.
## Call `lifecycle::last_warnings()` to see where this warning was generated.p_combined <-
plotly::subplot(p2,p1, # plots to combine, top to bottom
nrows = 2,
heights = c(.6,.4), # relative heights of the two plots
shareX = TRUE, # plots will share an X axis
titleY = TRUE
) %>%
# create a vertical "spike line" to compare data across 2 plots
plotly::layout(
xaxis = list(
spikethickness = 1,
spikedash = "dot",
spikecolor = "black",
spikemode = "across+marker",
spikesnap = "cursor"
),
yaxis = list(spikethickness = 0)
)## Warning: Ignoring 1 observationsp_combinedSave the plot to pull into the index
save(p_combined, file = "./plotly_fig.rda")Save an htmlwidget for website embedding
htmlwidgets::saveWidget(p_combined, "plotly_fig.html")Build loess smoothing figures figures
#create smoothing data frames
#n1
smooth_n1 <- only_n1 %>% select(-c(Facility)) %>%
group_by(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke, cases_per_100000_clarke) %>%
summarize(sum_copy_num_L = sum(mean_total_copies)) %>%
ungroup() %>%
mutate(log_sum_copies_L = log10(sum_copy_num_L)) %>%
mutate(target = "N1")## `summarise()` regrouping output by 'date', 'cases_cum_clarke', 'new_cases_clarke', 'X7_day_ave_clarke' (override with `.groups` argument)#n2
smooth_n2 <- only_n2 %>% select(-c(Facility)) %>%
group_by(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke, cases_per_100000_clarke) %>%
summarize(sum_copy_num_L = sum(mean_total_copies)) %>%
ungroup() %>%
mutate(log_sum_copies_L = log10(sum_copy_num_L)) %>%
mutate(target = "N2")## `summarise()` regrouping output by 'date', 'cases_cum_clarke', 'new_cases_clarke', 'X7_day_ave_clarke' (override with `.groups` argument)#add trendlines
#extract data from geom_smooth
#n1 extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_n1 <- ggplot(smooth_n1, aes(x = date, y = log_sum_copies_L)) +
stat_smooth(aes(outfit=fit_n1<<-..y..), method = "loess", color = '#1B9E77',
span = 0.6, n = 134)## Warning: Ignoring unknown aesthetics: outfit#n2 extract
extract_n2 <- ggplot(smooth_n2, aes(x = date, y = log_sum_copies_L)) +
stat_smooth(aes(outfit=fit_n2<<-..y..), method = "loess", color = '#1B9E77',
span = 0.6, n = 134)## Warning: Ignoring unknown aesthetics: outfit#look at the fits to align dates and total observations
#n1
extract_n1## `geom_smooth()` using formula 'y ~ x'
fit_n1## [1] 11.07337 11.23133 11.38495 11.53368 11.67693 11.81416 11.94480 12.06829
## [9] 12.18474 12.29492 12.39927 12.49822 12.59221 12.68168 12.76705 12.84669
## [17] 12.91899 12.98440 13.04338 13.09637 13.14384 13.18625 13.22404 13.25768
## [25] 13.28762 13.31432 13.33822 13.35980 13.37950 13.38823 13.37842 13.35280
## [33] 13.31410 13.26505 13.20838 13.14682 13.08310 13.01996 12.96012 12.90631
## [41] 12.86127 12.82772 12.80839 12.79060 12.76303 12.73048 12.69771 12.66954
## [49] 12.65073 12.64608 12.65363 12.66774 12.68770 12.71279 12.74229 12.77547
## [57] 12.81162 12.85002 12.88994 12.93066 12.97148 13.01166 13.05048 13.08723
## [65] 13.13082 13.18895 13.25910 13.33872 13.42528 13.51625 13.60909 13.70127
## [73] 13.79025 13.87351 13.94849 14.01268 14.06354 14.09853 14.12572 14.15365
## [81] 14.18047 14.20436 14.22346 14.23594 14.23997 14.23245 14.21265 14.18225
## [89] 14.14294 14.09640 14.04433 13.98841 13.93032 13.87175 13.81439 13.75992
## [97] 13.71004 13.66642 13.63075 13.59809 13.56277 13.52539 13.48654 13.44683
## [105] 13.40685 13.36719 13.32846 13.29124 13.25614 13.22376 13.19469 13.16952
## [113] 13.14886 13.12992 13.11009 13.09021 13.07111 13.05364 13.03864 13.02693
## [121] 13.01797 13.01062 13.00489 13.00080 12.99833 12.99750 12.99831 13.00077
## [129] 13.00488 13.01064 13.01807 13.02716 13.03791 13.05035#n2
extract_n2## `geom_smooth()` using formula 'y ~ x'
fit_n2## [1] 10.85262 11.04904 11.24008 11.42528 11.60418 11.77632 11.94124 12.09848
## [9] 12.24811 12.39074 12.52675 12.65647 12.78028 12.89854 13.01160 13.11816
## [17] 13.21693 13.30828 13.39257 13.47016 13.54141 13.60669 13.66637 13.72079
## [25] 13.77034 13.81537 13.85625 13.89333 13.92699 13.94907 13.95282 13.94070
## [33] 13.91517 13.87869 13.83373 13.78275 13.72821 13.67257 13.61830 13.56786
## [41] 13.52370 13.48830 13.46411 13.43359 13.38313 13.32109 13.25586 13.19579
## [49] 13.14927 13.12467 13.11573 13.11046 13.10862 13.10998 13.11428 13.12130
## [57] 13.13078 13.14249 13.15619 13.17164 13.18859 13.20681 13.22605 13.24608
## [65] 13.27364 13.31417 13.36549 13.42538 13.49164 13.56207 13.63446 13.70661
## [73] 13.77633 13.84140 13.89962 13.94879 13.98672 14.01118 14.02748 14.04219
## [81] 14.05506 14.06584 14.07429 14.08016 14.08320 14.08030 14.06929 14.05126
## [89] 14.02731 13.99856 13.96611 13.93107 13.89454 13.85762 13.82143 13.78706
## [97] 13.75563 13.72824 13.70599 13.68558 13.66326 13.63942 13.61451 13.58892
## [105] 13.56309 13.53742 13.51234 13.48827 13.46562 13.44481 13.42626 13.41038
## [113] 13.39761 13.38617 13.37437 13.36269 13.35160 13.34159 13.33313 13.32670
## [121] 13.32198 13.31831 13.31569 13.31411 13.31357 13.31407 13.31560 13.31816
## [129] 13.32175 13.32637 13.33201 13.33867 13.34634 13.35503#assign fits to a vector
n1_trend <- fit_n1
n2_trend <- fit_n2
#extract y min and max for each
limits_n1 <- ggplot_build(extract_n1)$data## `geom_smooth()` using formula 'y ~ x'limits_n1 <- as.data.frame(limits_n1)
n1_ymin <- limits_n1$ymin
n1_ymax <- limits_n1$ymax
limits_n2 <- ggplot_build(extract_n2)$data## `geom_smooth()` using formula 'y ~ x'limits_n2 <- as.data.frame(limits_n2)
n2_ymin <- limits_n2$ymin
n2_ymax <- limits_n2$ymax
#reassign dataframes (just to be safe)
work_n1 <- smooth_n1
work_n2 <- smooth_n2
#fill in missing dates to smooth fits
work_n1 <- work_n1 %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_n1 <- work_n1$date
work_n2 <- work_n2 %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_n2 <- work_n2$date
#create a new smooth dataframe to layer
smooth_frame_n1 <- data.frame(date_vec_n1, n1_trend, n1_ymin, n1_ymax)
smooth_frame_n2 <- data.frame(date_vec_n2, n2_trend, n2_ymin, n2_ymax)#make plotlys
#plot smooth frames
p3 <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_n1, y = ~n1_trend,
data = smooth_frame_n1,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_n1,
'</br> Median Log Copies: ', round(n1_trend, digits = 2),
'</br> Target: N1'),
line = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
plotly::add_lines(x = ~date_vec_n2, y = ~n2_trend,
data = smooth_frame_n2,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_n2,
'</br> Median Log Copies: ', round(n2_trend, digits = 2),
'</br> Target: N2'),
line = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
plotly::add_ribbons(x ~date_vec_n1, ymin = ~n1_ymin, ymax = ~n1_ymax,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_n1, #leaving in case we want to change
'</br> Max Log Copies: ', round(n1_ymax, digits = 2),
'</br> Min Log Copies: ', round(n1_ymin, digits = 2),
'</br> Target: N1'),
name = "",
line = list(color = '#1B9E77')) %>%
plotly::add_ribbons(x ~date_vec_n2, ymin = ~n2_ymin, ymax = ~n2_ymax,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_n2, #leaving in case we want to change
'</br> Max Log Copies: ', round(n2_ymax, digits = 2),
'</br> Min Log Copies: ', round(n2_ymin, digits = 2),
'</br> Target: N2'),
name = "",
line = list(color = '#D95F02')) %>%
layout(yaxis = list(title = "Total Log SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
plotly::add_segments(x = as.Date("2020-06-24"),
xend = as.Date("2020-06-24"),
y = ~min(n1_ymin), yend = ~max(n1_ymax),
opacity = 0.35,
name = "Bars Repoen",
hoverinfo = "text",
text = "</br> Bars Reopen",
"</br> 2020-06-24",
showlegend = FALSE,
line = list(color = "black", dash = "dash")) %>%
plotly::add_segments(x = as.Date("2020-07-09"),
xend = as.Date("2020-07-09"),
y = ~min(n1_ymin), yend = ~max(n1_ymax),
opacity = 0.35,
name = "Mask Mandate",
hoverinfo = "text",
text = "</br> Mask Mandate",
"</br> 2020-07-09",
showlegend = FALSE,
line = list(color = "black", dash = "dash")) %>%
plotly::add_segments(x = as.Date("2020-08-20"),
xend = as.Date("2020-08-20"),
y = ~min(n1_ymin), yend = ~max(n1_ymax),
opacity = 0.35,
name = "</br> Classes Begin",
"</br> 2020-08-20",
hoverinfo = "text",
text = "Classes Begin",
showlegend = FALSE,
line = list(color = "black", dash = "dash")) %>%
plotly::add_segments(x = as.Date("2020-10-03"),
xend = as.Date("2020-10-03"),
y = ~min(n1_ymin), yend = ~max(n1_ymax),
opacity = 0.35,
name = "</br> First Home Football Game",
"</br> 2020-10-03",
hoverinfo = "text",
text = "First Home Football Game",
showlegend = FALSE,
line = list(color = "black", dash = "dash")) %>%
plotly::add_markers(x = ~date, y = ~log_sum_copies_L,
data = smooth_n1,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Observed Log Copies: ', round(log_sum_copies_L, digits = 2)),
marker = list(color = '#1B9E77', size = 6, opacity = 0.65)) %>%
plotly::add_markers(x = ~date, y = ~log_sum_copies_L,
data = smooth_n2,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Observed Log Copies: ', round(log_sum_copies_L, digits = 2)),
marker = list(color = '#D95F02', size = 6, opacity = 0.65))
p3Create final trend plot by stacking with epidemic curve
smooth_extract <-
plotly::subplot(p3,p1, # plots to combine, top to bottom
nrows = 2,
heights = c(.6,.4), # relative heights of the two plots
shareX = TRUE, # plots will share an X axis
titleY = TRUE
) %>%
# create a vertical "spike line" to compare data across 2 plots
plotly::layout(
xaxis = list(
spikethickness = 1,
spikedash = "dot",
spikecolor = "black",
spikemode = "across+marker",
spikesnap = "cursor"
),
yaxis = list(spikethickness = 0)
)## Warning: Ignoring 1 observationssmooth_extractsave(smooth_extract, file = "./smooth_extract.rda")